I need help! Is there a function which excludes images with low child object identification from analysis?
I'm looking at child primary objects within parent primary objects (metaphase chromosomes). My child primary objects usually number around ~160 per metaphase spread.
What I've realized is that my pipeline can't distinguish the metaphase chromosomes when they happen to be close together AND overlapping with a nuclei.
The result of these circumstances is usually 1-4 chromosomes identified, <20 child primary objects identified, and the rest of the chromosomes not identified. Is there a way to exclude these images, which haven't properly identified all chromosomes (and thus the child objects), by a pipeline function?
Thanks so much for reading.. I'm brand new to this software and forum! Not sure what other information to include.. cheer!