I am just a junior for this software,it is a very well software to do some analysis. And now I am working with analysis DCX-labeled cells in the brain. Please see the attachment about this staining.
I have two factors need to analysis: 1.is the number of DCX-labeled cells ; 2. is cell layer thickness of DCX-labeled cells. And I establish a quite simple pipeline for calucate the cell numbers.
It is the pipeline:
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The threshlod that I set up for Hoechst staining is [0.05,1]; for DCX staining is [0.2,1];
The diameter for Hoechst staining is [4,10]; for DCX staining is [5,20].
This pipeline look works for calculat the cell numbers, but the problem is: the DCX-staining isnot specific for nuclear or cytoplasm, the labeled cell has a long dentrites projection from cell body.So when i do analysis, the projection was also recognized as cells.So that could be increase aritfical error of analysis.I tried crop module,it looks works not very well. So could you give me some suggestions about how to anyalsis the cell body’s number and delete the signal of projections? Thank,
At the mean time, about how to analysis the layer thickness, i absolutely have no idea of which module I need to created. Just give me a clue. I will appreciate about that. Thanks very much!!