I’m trying to analyze incell analyzer images for cardiomyocite morphology study. I want to get information about the nuclei, cell itself and actin at this point. Cell are stained with DAPI, Cyt3, Cyt5 and FITC. I’m using the the pipline shown in the link below, and images below. DAPI gives information about the nuclei (thus number of cells) and FITZ about actin(thus I can get information regarding the cell boundary, and also cell shape in general). However, DAPI and FITZ images are separate and makes identifying primary, secondary and tertiary objects difficult.the pipeline pciks onlyone image(in this case DAPI image), and identify the secondary and tertiary object based only on this image. My question: Is it possible to merge two and or more images (in this case DAPI and FITZ images) to get information regarding the nucleus(cell number) and actin(cell boundary & morphology)?
Acurate_segmentation.cppipe (11.6 KB)
Thank you in advance fro your help.