I have just entered the Cellprofiler community and I am getting familiar with the Cellprofiler.
I would like to count DAPI-stained cells (nuclei) from the confocal images (see attached) obtained from frozen heart sections by using threshold, object size and circularity (shape) criteria. A simple task for an expert, you may say… Could anyone have a look at the attached pipeline and perhaps guide me step by step to set the correct settings.
A similar problem was posted by shupadh, but I couldn’ make it work with my images…
TUNELPIPE_simon.mat (939 Bytes)