I have Z stacks, each with about 80 planes. Overall, the stacks capture spherical balls of cells. In order to try to assess the volume of these balls of cells, I’d like to try to measure the X-Y area of the ball in each slice of the Z stack. Do you know if there is any way to accomplish this in CellProfiler? Essentially for each frame of the Z-stack, I’d like to apply some thresholding and intensity modifying steps, and then I’d hope to use something like the IdentifyPrimaryObjects module to identify the boundary of the ball of cells. Then I’d hope to repeat that for each frame of the Z-stack. Do you know if this is possible?