We in the Imaging Platform at Broad love to hear from you and help you! However our time is limited. (Especially for free )
So if you are requesting help regarding your assay, do the following:
Check the FAQ
to see if your problem has been addressed already. - Use the Search function (box at the upper-right corner, or the Advanced search here
) to see if other users have asked the same question and received an answer.
If you choose to create a new topic, please attach to your post:[list]
*]A description of the phenotype of interest, i.e, the cellular image features that you are looking for. The team has biological experience, but we may not be fluent in the details of your assay. A basic novice description goes a long way to making sure that we understand what needs to be done.
At least one sample image (or set of images that comprise a minimal data set). Better yet, an example of both a positive and a negative control.
A CellProfiler pipeline (i.e. a *.cp file). At least make an attempt!