Please carefully check the channels after you convert ColorToGray. We aim to have Lipid droplet in the OriGreen channel, protein in OriRed channel and OriBlue for nuclei.
The screenshot you uploaded shows a wrong match - up.
Once you have the correct channels, the EnhanceOrSuppressFeatures can then help each components stands out: for example here is the nuclei
Here is the lipid droplets
Identify lipid droplets with correct channel
You can add MeasureObjectSizeShape to measure the sizes of lipid droplets in pixel unit.
To convert from pixels to micrometer:
- Try to check with ImageJ > Image Properties > to see the pixel size of your image.
- If your images don't contain this information, you would need to look at the settings of your microscope (the software that accompanies and control your microscope). It may also be termed "configuration" or "calibration" .
For e.g. pixel size = 0.245 x 0.25 micronbodipy.cpproj