I constructed a simple pipeline to load .flex files, separate them into GFP and RFP images, then save the independent images. Unless I put in a rescale intensity (stretch from 0-1) module (which I don’t want to do since I would like to be able to visualize any abundance changes between images), the images that CellProfiler saves are essentially void of any information. I’ve attached the pipeline I used, but haven’t attached the output image (it would just be an empty image)
Does this have anything to do with the bug described in the initial post? If so, is there anything I can do about it using the compiled CellProfiler application?
(I am running CellProfiler on a core duo macbook, OS 10.5.6 using the java workaround)
saveimagesPIPE.mat (698 Bytes)