so a basic question first: I’ve read that the mean background and presumably the mean signal as well is calculated by IntegratedIntensity/area. Is that correct? Does that mean you assume the pixel intensities to be linear (no gamma curve)?
If that is the case, shouldn’t I be getting the same results if I let CP calculate the mean signal compared to if I divide the sum of IntegratedIntensity by the area for the signals (per photo)? I don’t get the same values…
I noticed it when I wanted to calculate the signal without the background. Essentially:
Sum IntegratedIntensity per photo - (mean background * area signal)
Is there anything wrong with this approach?
Is there a way to get the area for each secondary object (instead of AreaOccupied)?