The phenotype I am trying to analyze involves cell to cell fusion of human cells. I would like to measure the degree of fusion shown in an image. In other words, I’d like to know the percentage of cells that are fused versus the percentage of cells that remain unfused ( or single cells). Could anyone suggest a pipeline or individual modules that would help with this? Much thanks!
It’d be helpful to see some images to know what they look like, have a sense of what you’re staining, etc; take a look at our tips for new posts to get a sense of the information we’d need to help you out. Thanks!
Thank you for the advice bcimini! I will post again with a better description and images.