I have a DAPI image which I did deconvolution on, this helped clear it up and the nuclei are a lot cleaner.
I am using CP to IDPrimary objects, however the identification is actually better before the deconvolution.
Does this make sense to you guys? I’m pretty puzzled about it, I’ve played around with the identification parameters but could not get the deconvolved image to be identified well.
Thanks for your reply