CellProfiler for Tissue Microarray Immunohistochemistry


#1

Hi,
Recently used CellProfiler and examples worked fine
Using Mac( intel) OS X Leopard
I need help in using CellProfiler as quantitative image analysis tool for the cropped or copied tissue core images
Immunohistochemistry is diaminobenzidine -peroxidase -based ( usually brown but sometimes red or blue color)
See sample Tma image attached.
The attached image is a small set of Tma. I am using immunoperoxidase-based immunohistochemistry to determine the level of stained “protein” in each tissue core. I previously used an


ImageJ plugin TMA color decon but the output is all brown intensity and individual cell content of “protein” not determined. I like the multiple features available in CellProfiler. I also like the fact it is automated.
Hoping image is OK


#2

Hi,

Have you checked out the Example Tissue Neighbors pipeline, and the Wound Healing Assay? both explain how to quantify staining in tissue monolayers.