CellProfiler not distinguishing between small droplets



As you can see from my image below cellprofiler appears to be grouping together smaller droplets, I was wondering if anyone could tell me how I might be able to get it to differentiate between these droplets better?

Thank you!


It’s hard to give accurate advice based on the information provided (see below), but in general you’d want to tinker with the options for how it’s declumping, how it’s drawing the dividing lines, if it’s suppressing them because they’re too close together- basically anything in the bottom half of the options in “Identify Primary Objects” (read the help file on that module for more information). Additionally, if you ONLY care about small objects and not the large ones, you could try to include an “EnhanceOrSuppressFeatures” module upstream to pull out objects of the size that you care about.