Hi Cell Profiler Community,
I have a cell line that faintly expresses my reporter gene. With a fancy scope I can get the signal pretty clear, but I am collecting images on a GE InCell 2200 and the attached image is what I get after re-scaling the intensity. From this point, I am not sure what I can do to better clean up the image. Before I had more distinction between background and objects so I could get away with some quick and dirty image math. Now I need a bit more finesse.
I can make out the signal so I figure, with the right amount of skill I should be able to clean this up right?
The intensity histogram shows a single hump Gaussian curve so I am not sure how to cleanly discern objects from background. Though since I can visibly see it sort of there has to be a way right?
Example image jpeg snip of Tiff file
Thanks in advance.
Updated so example image would show.