Hi, I have been trying to segment cells in a co-culture system but currently I am stuck on things which I should do in building the pipeline. I have already created a z projection on a separate pipeline, increased the intensity and also tried to pick up all nuclei by size. I have also applied a mask through and in the middle of trying to pick up the 2 individual population of cells by “identify primary object”. I believe after that I can perform a ground truth & supervised machine learning in CPA?
I just want to double check whether this is the right approach to co-culture analysis? I have this idea after completing the hepatocyte & fibroblast co-culture tutorial. If yes, I am wondering if anyone can guide me through how I can create the ground truth files and images for CPA? I am quite lost and would appreciate any comments or help! Many thanks!