Colocalization via two different channels



I am trying to identify nuclei that co-localize two different stains from two different channels. When I try to do this with the RelateObjects module, I end up getting a significant amount of false-positives for when two different stains are just adjacent to one another, rather than being perfectly superimposed. Is there a better way to go about this?



Glad you asked! We actually have an example pipeline and even a tutorial written for this task, here:

Take a look and please let us know if bits are outdated (even better, mark up a paper copy and send it to us so we can correct it!)