I'm currently an undergraduate doing research on L6 muscle cells that were incubated with 2-NDBG, a glucose uptake indicator, and were infected with a red fluorescent tagged T. gondii strain. My cells were stained with cellmask and gfp. I'm relatively new to using CellProfiler and I'm finding it difficult in making a pipeline to analyze my images.
Here are my pictures taken manually with Cytation 3. I know some of these images aren't the greatest:
Any help is greatly appreciated. Thank you!