I’m new to comet assay and I’m trying to use the comet assay pipeline in cell profiler to automate analysis.
The pipeline is able to identify the primary objects (comets).
However it is unable to demarcate the comet head.
Please suggest how to tweak the pipeline so that I can automate comet head identification.
Alternatively is the problem in my images and not the pipeline?
Any help would be appreciated.
I have uploaded some sample images.