Comet assay


#1

Hi all
First of all I would like to congratulate all of you on a fastastic piece of software. I have been using it on cell based fluorescence assays and it works very well. The other software I use is ImageJ which is also very good.
I wonder if there are any modules that allow the calculation of comet tail moments (comet assay or single cell gel electrophoresis assay) or associated parameters (comet tail lenght, %DNA in tail etc) because it would allow the processing of thousands of images I have accumulated. i am trying to set up a module to do this automatically using cellprofiler but i am new to programming…

Anyway, thanks a lot!


#2

Hello,
I am actually helping a collaborator build a pipeline for a comet assay. As far as I can tell, it is working well and will likely be posted on the CellProfiler website during the next release. I’ve found that 10x images work better than 20x images. If you would like me to take a look at one of your images, please email me at mvokes@broad.mit.edu

~martha