I created an illumination function using the module in the title above - it is here: IlluminationCorrection.cpproj (642.0 KB)
Illumination function - Background.
Rescale - No.
The output illumination function is a ‘.mat’ image. What do the various values for each pixels represent? Are they the absolute background intensities of my images? Or is it a percent/ fractional value?
For eg. most of the pixels of the illumination functions image are in the range 0.03 - 0.05. Is this value subtracted from my ‘sample’ images? Or is it 3-5% of the maximum or such?