Counting the number of Nuclei/cell and nuclear density in IHC image

nuclei
ihc

#1

Hello,

I am very new to Cellprofiler and I am attempting to count the number of nuclei/cell in my IHC image. I have converted the image to gray using the ColorToGrayModule, and then inverted the image using ImageMath.

I am attempting to identify the nuclei as my primary object, and cell border as my secondary object. The goal is to count the amount of DNA/cell (density) and number nuclei/cell. Is there a module that already does something like this?

Best,
Juan