I managed to create a working pipeline for measuring the csa of muscle’s fibers, but i noticed something strange. The pipeline work well in recognizing and counting the areas of every fiber, but the area in pixels, compared to the same area analysed previously with the csa imagej plug-in, is at least 3-4 time bigger.
I know from previous work that imagej is right and i suppose that in my pipeline, inadvertently, i implemented a module that enhance the pixel density for the image.
Or at least something similar.
Unfortunately, i can’t figure out which module is causing this problem and, due to this, i can’t implement a “correction factor” in my measurement.
Someone can give me some hint about this?
thank-you in advance
CSAworking20x_2.0.cpproj (411.4 KB)