Detection of Cell Patches in Cell Profiler


#1

Hello,

I’m new CellProfiler user and new member of this blog. Is it possible to detect cell patches in CellProfiler? Imagine we have 1000 cells randomly distributed in a square but 100 cells have been colonized somewhere among them. I would appreciate if someone gives me a hint if it’s possible to detect such an structure in CP. I’ve attached a schematic illustration of my cells here:

Thanks,
Payam


#2

Hello,

You can try to smooth or morph the image first, to make these patches become single dense objects, and perform segmentation after.

As shown here:


and here:

Bests,