Determining optimal settings for 'track objects'

migration

#1

Hi,
I’m trying to set up a single cell migration assay. Cells are labelled with strong nuclear label so segmentation is good. I am using the track objects module, and trying to determine optimal settings for consistent tracking of objects at a few different seeding densities.
Is there a way to scroll through the images in test mode to visually confirm that the label is correct across images while choosing ‘tracking method’?
Also, is there a way to overlay consecutive images to measure the ‘distance’ between frames?

help much appreciated!

Cheers


#2

Although we do not actively maintain it, and it’s only for Windows, there is a companion tool called CellProfiler Tracer. It does not meet all of your needs but may be helpful: http://cellprofiler.org/tracer/

You could also check out the paper where that tool is described: https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-015-0759-x