I’m trying to set up a single cell migration assay. Cells are labelled with strong nuclear label so segmentation is good. I am using the track objects module, and trying to determine optimal settings for consistent tracking of objects at a few different seeding densities.
Is there a way to scroll through the images in test mode to visually confirm that the label is correct across images while choosing ‘tracking method’?
Also, is there a way to overlay consecutive images to measure the ‘distance’ between frames?
help much appreciated!