Discard the no-GFP cells


I need help again… I treated the cells with GFP transfection and stain the nuclei with Hoechst. I found a lot of cells without GFP and I would like to discard these cells. How can I do that? Thanks.


You can use the ClassifyObjects module to classify different groups of GFP expression (either high GFP or little/no GFP). Alternatively, you can use the CalculateRatios module to calculate the ratio of GFP to Hoechst. You can easily ‘discard’ the non-transfected cells during your analysis, rather than during the image processing. (This will allow you to later calculate transfection efficiency.)