Error = exception in handling display request for module tertiary objects #7


#1

One of our Facility users is trying to analyse images from a 96-well plate and his pipeline keeps crashing out on the following error: exception in handling display request for module tertiary objects #7
Unfortunately it always seems to happen after running just fine for a large number of images (>300) and in the middle of the night. It doesn’t seem to a particular image it doesn’t like, and the offending ones open fine afterwards in ImageJ or Photoshop. I was wondering if anyone has any suggestions as to what might be causing this error and how I can solve it for him? Pipeline is simple: find nuclei (primary), identify cell (secondary) and cytoplasm (2-1= tertiary).
Thanks.
Hanneke


#2

It may be as simple as being a memory error- is the “eyeball” next to IdentifyPrimaryObjects open or closed? If open, can you try closing it (even better, go to Window-> Hide all windows on run) before running it?