I am new to CellProfiler and I have just started using it to analyse my live imaging experiments.
I created a pipeline to recognize for every image the number of dots in the green channel, but when I run the analysis on my image set, which is composed of nearly one thousand pics - divided into the three channels, I receive the following message:
I don´t understand what is the issue. Here is the pipeline:
MS2_Speckles_v3.cppipe (18.7 KB)