Error on "Export to Excel"


#1

I get the following error message when I attempt to import ObjectIntensity measurements to Excel:
"Measurements from a previous version of CellProfiler detected. Processing of this data may require using the same version of CellProfiler that created them. Should an attempt be made to read them anyway?"
I am only using the newest version of CellProfiler (on a new computer, with a new Pipeline), so don’t understand why the message appears.
If I answer “Yes” to the query, the data does get exported (but imperfectly, without object ID#s.
Thanks for your help.


#2

I notice that someone else has noted this problem.
I checked to see what module files my “preferences” were pointing to, and it is what seems to be the correct version: created 11/5/08.


#3

Hi,

Would you mind uploading your pipeline to the forum so we can check it out? If you have an image that we can run it with, please upload that too.
-Mark


#4

Hi Mark -
The error comes when I try to export data from the MeasureObjectIntensity module.
It exports OK when I use data from MeasureObjectAreaShape module.
I am attaching the pipeline and one of the source files.
The other one is apparently too large to upload so I’m sending it separately.
Thanks,
ibv



Analyze and exportPIPE.mat (867 Bytes)


#5

Hi Bert,

It seems that the warning is appearing for exactly the reason it says it is: There are old measurements that are being exported because the intensity measurements generated by SubtractBackground were never updated for the newest release! This is a bug that we will fix.

To deal with this, I recommend using CorrectIllumination_Correct and _Apply instead. SubtractBackground is a fairly esoteric module, one which we don’t use much ourselves. The pipeline which I am attaching to this post has the CorrectIllumination modules added with settings to give results much as SubtractBackground did (IDPrimAuto found the same number of objects in both cases).

Regards,
-Mark
Analyze and exportPIPE2.mat (1.14 KB)