Fetches wrong image in CPA


#1

Hello, when I fetch my image in the image gallery in CPA, the program only fetches my black and white image and not my colored image. How do I get it to pull up my colored image? Just a little background, I am trying to score TUNEL stained slides so I need the colored image to tell the program what is a positive and what is a negative nuclei. I can’t do that with a black and white photo since the positive nuclei are stained brown and the negative nuclei are stained blue/green. I need to know how many positive nuclei and negative nuclei there are in each image.