Hi CP team,
I’ve been reading previous posts and also the help section (?) to understand how the filter object works. I’m interested in filtering by intensity but I am a bit confused and wondering if you can explain a bit more or have an example to illustrate?
I want to use this in my co-culture imaging assay to eliminate the false positive stained cells. (The CTRL are on either side of the plates i.e col 2 & 23 in a 384well plate) A draft of my pipeline is as below and it would be great if you can comment on it and if I should include any other steps?
Do I also need to have another pipeline setup to calculate my CTRL intensity first? or can I have them together in one & how?