H&E cell counting


Hi everyone,

I managed to use cellprofiler to count the cells on an H&E stained tissue but unfortunately I can’t separate the cells of the extracellular matrix. Also I have to dissociate my tissue from some big particles and I wasn’t able to do it.

I’ve already tried the pipelines in different topic on the forum but It didn’t work efficiently for me.

Can anyone help me ?

Thank’s in advance


We can certainly try, but we need more information first.