Thank you, Minh! It worked now. I did not chose the inverted version. Now I face two more problems. First one is about memory I think.
. I do have 8 gb of Ram so I don't know what is the problem.
The second problem is about one of RunImageJ function. I am curently trying to split channels of a TIF and to chose the Blue one. In ImageJ program there is a function called Split channels that let me split the channels and to chose the one that I want ( blue, red, green). I tried using Macros and Command but I did not get any results. I will show you a pic from ImageJ program.
This is what I hope to achieve in this program. I need the blue channel TIF so I could extract the background.
I am grateful to you for your help.