Thanks for the image.
It's in fact a difficult case when you have a noisy background and a low resolution/magnification at the same time.
Although I've tried a series of modules to reduce noise, it's quite unworthy to quantify this set of image
I highly recommend you thoroughly clean the sample after incubation of antibodies, because non-specific binding can dramatically reduce the image quality.
Also if possible, please capture the image with higher magnification.
I share my pipeline here to demonstrate the effort of cleaning the background fj test.cpproj (863.2 KB)