I am counting cilia (which may appear as tiny dots or thin string like objects). The cilia are in Cy3, while the dapi are blue. Unfortunately, the antibody for cilia sometimes binds non-specifically, resulting in random dots that look like cilia, but aren’t cilia (we know they aren’t cilia since they are not near a dapi object). The cilia should be no more than 15 microns from a dapi object, so I am wondering if there is a way to tell the program to only count cy3 objects that are no more than 15 microns from a dapi object.
We have told the program already how to identify cilia objects and dapi objects, but due to the non-specific cilia antibody staining, we want the program to only count the cy3 objects that are 15 microns from dapi.
Thanks for the help!