recently i had to take .nd2 picture with channel FITC and DAPI even tough i have zero experience in processing these type of files. I tried to search on the forum to learn the rope about it, but couldn’t find much.
Specifically, these images are myotubes in fitc and nuclei in DAPI.
Before i managed to process this kind of images when in tiff format and split.
Someone can explain to me the basics to .nd2 files processing?
Thank you in advance.