I’m trying to identify dead cells in the Cy5 channel. Those are colored on top of the nuclei, therefore I’m using the IdentifyPrimaryObjects module. This works fine if there are dead cells in the image, however, those are very rare, and therefore I’m getting random signals identified (there are no dead cells) on the other images.
After having identified those dead cells, I would like to exclude those objects from the further analysis (preferably automatically for all channels). Is there a way to do this (e.g. get the object ids and exclude them from further analysis)?
Thank you very much for any assistance