I am currently using Cell Profiler for the first time. I am trying to identify and count double-labeled and triple-labeled cells. The images were taken on a confocal at 20X magnification and the labels are Zenk, BrdU, and Hu. I am trying to count the double-labeled Zenk and BrdU cells, the double-labeled BrdU and Hu cells and the triple-labeled Zenk, BrdU, and Hu cells. I figured out how to identify and count the cells separately using the “IdentifyPrimaryObjects” pipeline where I would input the images from each laser separately (so an image of just BrdU, just Hu, and just Zenk) and then counted the cells from each image. However, I am no unsure as to how to count the cells that are contained in multiple images. Can anyone please help me with this? Thanks!