I have an image with nuclei very close to each other (Image: https://files.fm/u/b9aqrdmh). I have rescaled the intensity and applied the threshold. The primary objects are being identified as clumps. Any suggestions? Is there a way of identifying primary objects using the module and then manually adding and editing objects as necessary? EditObjectsManually module does not allow me create objects and editing the boundaries also seems difficult by moving the circles.
Any help would be appreciated.