IdentifyPrimaryObjects identifies too many objects



I am a new user.

I am trying to do nuclear foci with stains of DAPI and Green (for my protein). In the IdentifyPrimaryObjects, my pipeline identifies too many objects in the green channel. I changed Threshold strategy and it does not make any difference.

h2ax-foci-count_1.cpproj (708.2 KB)

I would like to keep the number of objects identifies same as the number of foci i can see .

I am attaching my pipeline.