I tryed to count immunohistochemically stained (DAB not fluorescence) neurons in brain slices. The Problem is that the background within the slice is not always equal and that the staining intensity sometimes differs. Is there a module which identifies objects (e.g. neuron) relatively to it’s respective surrounding (and not a general threshold)?
E.g: A neuron has a diameter between 8 and 20 pixels and is 0.2 to 0.3 values darker (or brighter, if inverted) than it’s surrounding.
thanks for your help!