Invert image question


I have a 2D brightfield iPSC colony segmentation pipeline that I made which includes a bubble masking step. This masking requires using an invert image operation that used to be in the CellProfiler 2 morph module. I just noticed that it was probably removed because it duplicated functionality which is still supported in the ImageMath module.


Hi Derek,

Yes, that’s exactly what happened! Sorry for the confusion.