I have been able to successfully quantify total E-cadherin intensity, but am interested in quantification of junctional and cytoplasmic E-cadherin differentially, to establish a ratio of junctional to cytoplasmic E-cadherin intensity. I would be very interested in any recommendations or references you could offer for optimization of pipeline modules for this specific application.
Can you share you share a couple of your images with me?
of course, what format would be best?
you can upload image in tif format.