Junctional and cytoplasmic protein quantification


#1

I have been able to successfully quantify total E-cadherin intensity, but am interested in quantification of junctional and cytoplasmic E-cadherin differentially, to establish a ratio of junctional to cytoplasmic E-cadherin intensity. I would be very interested in any recommendations or references you could offer for optimization of pipeline modules for this specific application.


#2

Hi,

Can you share you share a couple of your images with me?

Regards
Hamdah


#3

of course, what format would be best?


#4

Hi,

you can upload image in tif format.

Regards
Hamdah