Legitimacy of Integrated Intensity quantification following "Enhance or Suppress Features"


I’ve been using “Enhance or Supress Features” in order to get foci within nuclei to stand out better which has greatly increased my ability to identify them as objects. However, I’m interested in how bright those foci are following different treatments and I’m concerned that the processing that goes on behind “Enhance or Suppress Features” may be incompatible with quantification.



I agree, I wouldn’t use the Enhanced image to quantify! I’d find where the foci are using the enhanced image, but then I’d measure the intensity in the MeasureObjectIntensity on your original image. Does that make sense?


Oh right that makes sense. Thanks!