I’m running CP 10415 on a Mac and try to load images of the following file structure:
— Hoechst_ND_50%B - n000000.tif
— Hoechst_ND_50%B - n000001.tif
— GFP_ND_50%B - n000000.tif
— GFP_ND_50%B - n000001.tif
— mKate_ND_50%B - n000000.tif
— mKate_ND_50%B - n000001.tif
The images are 16 bit grayscale images. Running the module everything seems to be fine. Even the “Choose image cycle” shows nicely that for each cycle the three corresponding images are recognized. But when I add a RescaleIntensity module for one channel it looks like there are multiple channels withing one image… Am I doing anything wrong?
I need to work with this older version because I then can use our cluster to run the pipeline later.
example.cp (2.41 KB)