SOX2_NuclearIntensity.cpproj (665.9 KB)
The attached pipeline is able to analyse most images without a problem However, some of the images I have tried to analyse produce an error (local variable 'rescaled_image' referenced before assignment). I have checked the pipeline and there are no references to the rescaled image before it is calculated in the RescaleIntensity module. I have tried copying the problem images to other folders but they still produce the same error.
The attached pipeline contains one image set (well E05) that works fine in the pipeline and one that doesn't (well G06). The problem images were all acquired on the same day.
Can anybody help me figure out what is going wrong and how to fix it?
Thanks for your help,