Haha wow sometimes its the most obvious things you dont think about. thankyou.
Another question. When I now run this I want to identify primary and secondary objects. What happens is that the z-stack is created gradually so lets say I have 15 z-slices.
it will analyse slice 1 and identify primary and secondary objects. then it will make stack 1+2 and identify primary and secondary then it makes stack 1+2+3 and so on.
I only need to identify objects once the entire z-stack has been created but do not see how to make this command.
Please let me know if this is possible.
(updated current protocol is attached)cell profiler pipeline.cpproj (142.5 KB)