I am trying to identify PLA dots in a noisy image (L20_dots.tif https://drive.google.com/open?id=1MhJauQmmGLNSy2O-jilMBh7tgyV6ajWx).
I can’t come directly to a satisfying result with IdentifyPrimaryObjects.
Here is my pipeline:
tresholding_manually.cpproj (473.3 KB)
So I first Enhance speckles with the EnhanceOrSupressFeatures.
Then I want to threshold the resulting Image before identifying the dots.
This is where I have an issue: I don’t manage to threshold the image like I would do in ImageJ (see picture below).
Even by reading previous posts on tresholding I can’t find the adequate value between 0 and 1 to perform a manual threshold that would match the result of what I could perform similarly to the 98.2% value used with ImageJ. I just get a back image at the end.