I demonstrate here a pipeline to extract the fibrotic region. Fibrosis.cpproj (642.6 KB)
The ideas are:
- Use module "UnmixColors" to separate 3 different territories: liver tissue, blood vessels, and fibrotic regions.
- With unmixed channels, identify object "liver tissue" and "vessels" accordingly.
- Use module "MaskImage" to extract fibrotic regions from the original picture.
Afterward, you can then measure intensity of the entire fibrotic region with module "MeasureImageIntensity"
Or identify these regions as objects first and then use module "MeasureObjectIntensity" (recommended, since you can also measure the size and area occupied)