I am wondering if there is a way to make measurements by ‘walking’ around an object. We would like to measure signal intensity at the cell cortex. I can create an appropriate object by finding the edge of the cell and contracting the object. As far as I can tell, the only measurements available are on a per object basis. Any variation in the object will be masked. I guess I could measure standard deviation or texture measurements of the object, but what I would really like to do is to divide the object into x segments of equal area, take the mean of these areas and plot them in a line graph.
Is there anything currently in CellProfiler that could help me do this?