Measuring Cell Fluorescence


Hi all,

I am new to cell profiler. I’d like to be able to measure the fluorescence of individual cells. I am using a single channel. All I’d like to be able to do is measure the change in intensity over time from a stack of tifs. How would I do this?



Have you used CellProfiler before? If not, a good place to start is resources on the website, specifically the tutorials and example pipelines. Sounds like the example using time-lapse data will be pertinent in this case.

Once you’ve started working on a pipeline, if you continue to have questions please feel free to post in the forum again and hopefully someone can give you more specific help.

Good luck!